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Human gammaherpesvirus 4 (HHV-4)
Human gammaherpesvirus 4 (HHV-4)
規(guī)格:
貨期:
編號:B162846
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human gammaherpesvirus 4 (HHV-4)
商品貨號 B162846
Classification Herpesviridae, Lymphocryptovirus
Deposited As Epstein-Barr virus
Agent Human gammaherpesvirus 4 (HHV-4)
Strain B95-8
Common Name Epstein-Barr virus
Applications Human and other primate B-lymphocyte transformation
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments

ATCC® VR-1492PQ™ is a high-titer, purified, live virus suspended in 10 mM Tris-HCL (pH 7.5) with 135 mM NaCl, 0.5% BSA, and 5% Trehalose. This product was prepared from ATCC® VR-1492™ via purification by tangential filtration.

This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™).

Quality control testing is done by transformation assay instead of TCID50.

Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Recommended Host
B95-8 cells
Growth Conditions

Temperature: 37°C

Recommendations for Infection: This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™).

Incubation: 10-25 days at 37°C in a humidified 5% CO2 atmosphere, until indicators of successful transformation appear.

Name of Depositor G Miller
Source Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
References

Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.

Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982

Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497

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胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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