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455
455
規(guī)格:
貨期:
編號:B163751
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 455
商品貨號 B163751
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with EGF receptors, but does not block the binding of EGF to the receptor on A-431 cells.
579, 225 or 528, see ATCC HB-8506, ATCC HB-8508 and ATCC HB-8509) to A-431 cells and binds with lower affinity than 528, 225 and 579.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor
Tumorigenic Yes
Effects
Yes, in pristane primed BALB/cJ mice
Comments
Animals were immunized with partially purified EGF receptors from A-431 cells.
Spleen cells were fused with NS-1-503 myeloma cells.
The antibody reacts with EGF receptors, but does not block the binding of EGF to the receptor on A-431 cells.
It does not inhibit the binding of other antibodies (e.g., 579, 225 or 528, see ATCC HB-8506, ATCC HB-8508 and ATCC HB-8509) to A-431 cells and binds with lower affinity than 528, 225 and 579.
It only marginally inhibits the growth of A-431 tumor cells in vitro, and does not block EGF stimulated phosphorylation of membrane proteins.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Regents of the University of California
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990

Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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