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71A7
71A7
規(guī)格:
貨期:
編號(hào):B163809
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 71A7
商品貨號(hào) B163809
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Con A molecule at a site distinct from the sugar binding site.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against concanavalin A (Con A)
Cellular Products
immunoglobulin; monoclonal antibody; against concanavalin A (Con A)
Comments
Animals were immunized with C.AL-20 (a T cell line) cells that had been incubated for 48 hours with 5 mcg/ml of Con A.
Spleen cells were fused with P3U1 myeloma cells.
The antibody reacts with the Con A molecule at a site distinct from the sugar binding site.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor FL Owen
Deposited As mouse (B cell); mouse (myeloma)
References

Spurll GM, Owen FL. Development of a cell line secreting monoclonal antibody specific for concanavalin A and use of that antibody as an affinity immunosorbent for tissue culture media used to support long-term T cell growth. J. Immunol. Methods 39: 335-341, 1980. PubMed: 6161967

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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