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AB1-2
AB1-2
規(guī)格:
貨期:
編號:B163902
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 AB1-2
商品貨號 B163902
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue lymph node
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain A/J
Applications
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
Genes Expressed
immunoglobulin; monoclonal antibody; against T15 idiotype
Cellular Products
immunoglobulin; monoclonal antibody; against T15 idiotype
Comments
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas.
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype mouse IgG1 kappa , IgG1; kappa light chain
Name of Depositor JF Kearney
Deposited As mouse (B cell); mouse (myeloma)
References

Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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