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Construct size (kb): 45.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3R*
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), regulatory subunit
(calcineurin B)
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M30773
Nucleotides 1-1836 of the insert correspond to
nucleotides 713-2548 of M30773.

Insert lengths(kb): 1.799999952316284

Tissue: brainstem

Gene product: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) [PPP3R*]

Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0, 0.35, 0.28 (doublet); BamHI--16.0, 11.0, 6.5 (doublet), 5.6; BglII--19.0, 15.0, 7.0, 2.4, 0.7;

EcoRV--5.6, 5.3, 4.5 (doublet), 3.7 (doublet), 3.3, 3.1, 2.5, 1.5, 1.4 (doublet), 1.3, 0.75, 0.6 (doublet); HindIII--21.0, 9.4, 6.0, 4.4, 3.8, 0.6; NdeI--20.0, 5.8 (doublet), 3.8, 2.4, 2.2, 1.7, 1.6, 0.55.

The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.61; EcoRI--0.27, 0.50, 1.57; EcoRV--0.53, 1.51; HincII--1.12; HindIII--0.13; NdeI--0.87; SphI--1.76.

Corresponds to nt 804-2548 of the GenBank record. It does not include the complete coding sequence (nt 759-1271).

Detects a 2.8-2.9 kb mRNA in rabbit brain, and a slightly smaller transcript in murine muscle and kidney.