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cMCOB5 [RW-202]
cMCOB5 [RW-202]
規(guī)格:
貨期:
編號:B241050
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 cMCOB5 [RW-202]
商品貨號 B241050
Designations cMCOB5 [RW-202]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
Isolated by a YN57 or 57 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 47.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D19S21]
Insert Size (kb) 40.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
BamHI--23.0, 17.0, 4.9, 2.3; EcoRI/HindIII--8.0, 4.9, 4.6, 4.1, 3.9, 2.5, 2.3, 2.0, 1.05 + smaller; matching depositors DNA.
EcoRI--8.0, 5.9 doublet, 5.1, 2.3, 1.8, 1.5; PstI--12.0, 8.0, 3.6 doublet, 2.2, 2.0, 1.25; HindIII--20.0, 14.0, 9.4, 3.7;
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Isolated by a YN57 or 57 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC).
References

Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Peter J O'Connell, personal communication

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